ABSTRACT
Bupleuri Chinensis Radix, as traditional Chinese medicine, has great use amount in the past dynasties, and the amount of the existing wild resources is not enough to provide market demand. In order to protect the wild resources and expand the source of medicinal materials, the research on the chemical composition, pharmacological action, clinical application and other aspects of Bupleuri Chinensis Radix and other plants in the same genus have been widely carried out. The paper reviews the study of medicinal plant resources of Bupleurum L. in China from the distribution and species, chemical composition of medicinal parts, resources protection and utilization of Bupleurum L. medicinal plants, etc, to provide new clues for Bupleurum L. resources development.
ABSTRACT
Objective: To study the effects of different pretreatment methods and culture temperatures on Seed Germination and seedling growth of Bupleurum chinense, in order to provide scientific basis for artificial cultivation of B. chinense. Methods: Several physiological indexes such as the weight, pure rate, content of moisture, the rate of water absorption per thousand seeds were measured. Adopting a double-layer filter paper culture method, the pretreated seeds were cultured in the incubator at 20 ℃ in the 40% light. In different culture temperature treatment groups, the seeds were soaked in distilled water 24 h and then cultured in the corresponding temperature incubator. The germination energy, germination percentage, germination index, vigor index of seeds, and the root length and the shoot height of the seedling were recorded. Results: The pure rate of seeds was 91.51%, the weight of one thousand seeds was (2.83 ± 0.03) g and the weight of seed after absorbing water was about 2.24 times more than the weight of naturally dried seed. The content of moisture of seeds was 7.3%. Different hormones had different effects on seeds, among them the effect of 0.6 mg/L 6-BA treatment was the best. Water bath could increase the seedling vigor index, and 40 ℃ of its temperature significantly promoted the seed germination and seedling growth. The germination rate of seeds was the highest under the condition of 15 ℃, but the root length and seedling height were lower. Conclusion: The suitable condition for seed pretreatment and culture temperature of B. chinense established in this research can effectively improve the germination rate and seedling rate, which is of great significance for seed breeding and artificial cultivation of B. chinense.